RPMI 1640 (RPMI) is another commonly used cell culture medium in biology, particularly for growing and maintaining lymphoid cells, such as white blood cells. Like DMEM, RPMI is a liquid mixture of nutrients, vitamins, and other substances that cells need to grow and divide. However, RPMI has a slightly different formulation than DMEM and is typically used to grow cells with different requirements. It has a higher concentration of essential amino acids and a lower pH, which makes it better suited for the growth of certain types of cells, especially hematopoietic cells.
Cell biologists may use RPMI to grow and maintain specific types of cells, such as white blood cells, in culture. RPMI is often used for studies involving the immune system, such as the study of leukemia, lymphoma, and other blood cancers. Additionally, it is often used as a culture medium for primary lymphoid cells, stem cells and for cell lines derived from lymphoid cells. It can also be used to study various aspects of cell growth and development, such as cell proliferation and differentiation.
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