Add water and microwave on thermocycle setting for 2-2.5 min. Enjoy your Instant PCR noodles! In biology studies, when you study a specific segment of DNA (more often a gene), a large amount (millions to billions copies of this DNA segment is needed for different kinds of studies. Here is what PCR (Polymerase Chain Reaction), lab technique for rapidly amplifying a specific segment of DNA, comes in to help. PCR was originally developed in 1983 by Kary Mullis, who was then awarded the Nobel Prize in Chemistry in 1993 for his invention.
A PCR reaction consists of 3 steps: denaturation, annealing and extension.
1) Denaturation is the separation of the double strand DNA. It happens by raising the temperature of the mixture.
2) Annealing happens when small DNA fragments called primers bind to the target DNA sequences and initiate polymerisation. This can only occur once the temperature of the solution has been lowered.
3) After primer annealing , with the help of a DNA polymerase enzyme, new strands of DNA are made using the original strands as templates. The cycle is repeated many times usually 20-30 times. It only takes 2–3 hours to get a billion or so copies.
The PCR products can used for DNA sequencing, for protein expression, for detecting the presence or absence of a gene to help identify pathogens during infection, and when generating forensic DNA profiles from tiny samples of DNA.
The "PCR Noodles Sticker" is a fun way to describe the PCR process. This sticker is printed on durable, high opacity adhesive vinyl, with beautiful glossy finish. The vinyl protects the sticker from scratches. Don't forget to clean the surface before applying the sticker.
• High opacity film
• Super durable vinyl, perfect for indoor use
• A perfect addition to any water bottle, journal, laptop, or wherever you like
• Size: 3'' x 3''